This year's conference will focus on imaging using MALDI mass spectrometry. An entire afternoon (Nov 29) will be dedicated to showcase such experiments in a workshop organized by Klaus Dreisewerd and his colleagues together with Waters Corporation at the Institute of Hygiene.

Prof. Dr. Ron Heeren, Maastricht MultiModal Molecular Imaging Institute (M4I), well known for his ground-breaking research in this field will elaborate on this topic in his keynote lecture on Nov 30. Other leading manufacturers in the field of MALDI imaging instrumentation and protein analysis - Bruker, PALL FortéBio, SCiLS and SERVA Electrophoresis will further illustrate the current state-of-the-art in talks and hands-on-workshops.This is an excellent opportunity for students and PIs alike to improve their understanding and discuss and troubleshoot with the experts.

We encourage you to use this great opportunity to train your students and meet the research leaders.

Fortebio

Biolayer Interferometry (BLI)

(November 29)

Venue: Seminar room Core Unit Proteomics (ZMBE 1st floor, Von-Esmarch-St. 56, side entrance ZMBE from the courtyard)

Abstract: Biolayer Interferometry allows rapid, real-time and label-free molecular interaction analysis. Many applications can be run on a single platform, from small molecule assays to large molecule-, liposome- and virus interaction analyses. Further, functional cellular responses can be detected by cBLI assays. Full kinetic characterization and protein quantification is possible, even from crude samples. The throughput is easily scalable from single to 96 parallel samples, the Dip-and Read approach allows sample recovery for additional experiments, and the microfluidics- free instrumentation requires little-to-no maintenance. BLI is the method of choice to replace time- consuming techniques like ELISA, Western Blotting or analytical HPLC.

10:30Workshop Registration
11:00Stefan Scory, Fortebio
Biolayer Interferometry (BLI) – a label-free, biosensor-based technology to monitor molecular interactions and signal transduction in living cells

Information is given on quantitative and kinetic analysis in sensitive affinity experiments
12:00 Lunch (not provided)

WORKSHOP Institute of Hygiene and Waters GmbH

MALDI imaging

(November 29)

Venue: Lecture hall (HS) at Robert‐Koch‐Str. 31 and laboratories at the Institute of Hygiene (Biomedical Mass Spectrometry) at Robert‐Koch‐Str. 41/51

13:00Workshop Registration
13:15Klaus Dreisewerd, Simone König
Welcome address
13:20Michael Batey, Waters Corporation, Wilmslow, UK
Molecular Imaging by Mass Spectrometry: Applications in Biomedical Research and Drug Discovery
14:20Klaus Dreisewerd, University of Münster
Brief introduction to MALDI‐MS imaging and MALDI‐2‐MS imaging
15:00Coffee break and lab tour in small groups
16:15Demonstration of experiments and data evaluation strategies via Team Viewer
  1. Simeon Vens-Cappell, University of Münster
    Enhanced MALDI‐MSI analysis of glycolipids from wild‐type kidney following on‐tissue enzymatic treatment with phospholipase C and use of ion mobility and tandem mass spectrometry
  2. Jens Soltwisch, Eike Ulrich Brockmann, University of Münster
    MALDI‐2‐MSI analysis of bacterial metabolites with a Synapt G2‐S
  3. Dennis Trede, SCiLS, Bremen
    Conceptual strategies for visualisation and analysis of mass spectrometry imaging data
18:00Closing remarks

TALK Bruker

MALDI Imaging

(November 30)

Abstract: The presentation will highlight Bruker´s latest developments in MALDI imaging mass spectrometry. Complementary MALDI imaging solutions based on either MALDI-TOF/TOF (Bruker rapiflex) or MALDI-FTICR (Bruker solarix 2xR) will be highlighted. The rapiflex MALDI-TOF/TOF technology allows to perform MALDI imaging at an unparalleled level of speed and spatial definition, whereas solarix 2xR facilitates MSI at incredibly high mass resolution and accuracy. The individual merits of both of these systems will be illustrated by application examples covering various research areas.

13:30 - 14:00
Arndt Asperger
Complementary MALDI imaging solutions based on latest MALDI-TOF and –FTICR MS technology

WORKSHOP Waters GmbH

Protein analysis

(November 30)

14:00 - 15:00
Marc Kipping, Waters GmbH
An Overview of Past, Present and Future of Data Independent Acquisition Strategies
(covers LC-MSe, Ion Mobiltity Spectrometry (IMS), HDMSe, Stepped Quadrupole, SONAR)


Introduction to modern mass spectrometry techniques also performed with your samples at the Core Unit Proteomics. A great opportunity to ask your questions about our experiments.
15:00 - 15:30
Gottfried Pohlentz, University of Münster
Efficient analysis of proteolytic peptides and glycopeptides by use of ion mobility separations (IMS) and subsequent low energy collision-induced dissociation

WORKSHOP Serva Electrophoresis

Protein electrophoresis for professionals

(November 30)

15:30 - 16:30Günter Thesseling, Reiner Westermeier, Serva Electrophoresis
Protein electrophoresis
(basics, overview of different techniques, visualisation of protein separation, trouble shooting)


The opportunity to learn about and discuss the beloved and hated workhorse in the biochemical laboratory, gel electrophoresis, with the experts and text book authors.